Antibody glycan characterization is an essential aspect of developing therapeutic antibodies, as the attached glycans can significantly influence an antibody’s stability, efficacy, and immune system interactions. Glycans, which are complex carbohydrates covalently bonded to the Fc region of antibodies, play a pivotal role in determining the antibody’s structural and functional properties, including its half-life and ability to engage with immune cells. Characterizing these glycans involves detailed analysis using techniques such as mass spectrometry, high-performance liquid chromatography (HPLC), and capillary electrophoresis. These methods help identify the glycan structures present and any variations that might affect the antibody’s performance. Through comprehensive Antibody Glycan Characterization, researchers can optimize the glycosylation profile during antibody production, ensuring consistent therapeutic activity and reducing immunogenicity risks in clinical applications.